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Spots showing a low signal-to-noise ratio or poor replicate consistency were discarded.
To avoid artifactual signals, samples and experiments with poor replicate quality were removed.
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Replicate libraries did not cluster together consistently, but this reflected the extremely minimal variance across the replicates rather than poor replication.
You have just a big division between wealthy and poor, which replicates itself in Brazil, Argentina, in Latin America in general".
Chips with poor biological replicates were discarded.
The reproducibility of experiments was qualitatively assessed on scatter plots by visual inspection and chips with poor biological replicates were discarded.
Even when that happens, measurements of the quality of individual datasets will remain important because they capture specific information in addition to reproducibility and because IDR analysis is sensitive to the presence of poor-quality replicates.
Despite the association between age and poorer prognosis replicated in these post hoc analyses, the overall trend for treatment benefit with decitabine was more clearly observed in patients aged 70 74 years and in patients ≥75 years compared with those <70 years.
Indeed, we have recently shown in mice that standardization may increase the incidence of spurious results in behavioral tests, accounting for poor reproducibility between replicate experiments, while systematic variation of experimental conditions (heterogenization) attenuated spurious results, thereby improving reproducibility [32].
Also, 50% DAR loss with our method of resection subjectively appeared to most closely replicate poor acetabular conformation in dogs with severe hip dysplasia.
The poor correlation of replicate values of the three male lung- versus-spleen arrays (Pearson R2 of 0.22 to 0.59) is likely because almost all of these values are close to zero, and variation is therefore predominantly experimental noise.
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