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Finally, poor quality staining can cause artefactual changes in the nature of the chromatin pattern.
In addition, we also evaluated the participant's option to decide whether some cases are inappropriate for EQA purposes, that some cases require expert opinion or there is either insufficient information or poor quality staining of the glass slides.
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A total of 612 (10.2%) slides had to be re-stained before a third reading was performed because of poor-quality staining.
Indeed, the reviewer is correct about the intracellular AcTUB staining, however, we do not believe this is due to poor quality of staining.
These would lead to poor and inefficient laboratory practice and has a negative impact on preparing quality stained slides.
Ambitious research projects that have not been adequately organized may suffer the consequences of poor quality or unreadable stains if slides are not used relatively quickly after sectioning.
Although BAL was taken from 12 asthma subjects, one sample had be eliminated due to very poor quality and non-specific staining.
As a consequence, early observations of nuclear PTEN were believed to be artifacts of poor quality antibodies used in immunohistochemical staining [10].
Figure 4: Most differentiation marker staining is of poor quality and hard to interpret.
Kiel staining was excellent for microdissecting CIS cells for DNA preparations [29], however the staining was generally very weak after 2 min staining and initial experiments showed a very poor RNA quality after staining with Kiel for 5 min (results not shown).
The staining was not evaluated due to poor quality of the sections in eight cases for Akt1, in 10 cases for Akt2 and in six cases for pAkt.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com