Sentence examples for poor quality regions from inspiring English sources
Recall that the quality score rewards the descending, smooth curves, and indeed STD has a smoother profile; however, RDN has the advantage of robustly locating poor quality regions (as discussed earlier).
All sequencing reads were trimmed of poor quality regions, and contaminating vector and linker sequences, and then assembled into contigs with Lasergene SeqMan Pro© version 8.0.2 software (DNASTAR , Inc.
Raw sequences were trimmed and cleaned using the Phred software [ 74], which resulted in the identification and removal of poor quality regions (quality cut-off of 20).
Thus, sequences from each group of strain-specific SSH libraries were trimmed, in order to exclude vector sequences and poor quality regions (Phred < 20), and aligned with the aid of CAP3 [ 74].
For each of the 6 strains, the sequenced clones have been trimmed, in order to exclude vector sequences and poor quality regions (Phred < 20), and aligned with the aid of CAP3, generating 1,063 contigs, which contained 6,712 sequences overall.
In several preprocessing steps, using pregap4 from the Staden package (19) and cross_match from phrap (http://www.phrap.com/), the raw EST reads were processed to clip all uninformative sequence parts, including poor quality regions, vector and adapter fragments, and poly-A/-T tails.
Poor-quality regions of the alignments were excluded using Gblocks version 0.91b [ 83] with relaxed parameters including the minimum number of sequences for a flank position (b2) set to 50%, minimum block length (b4) set to 5, and maximum number of species with gaps (b5) set to 50%.
We first excluded variants located in genomic regions of poor quality, which were defined as regions with read coverage in only one of a sample pair, probably due to randomness in sequencing process.
The poor quality of the region's public schools risks holding back the expansion of higher education (together with slow economic growth, that seems to be the problem in Mexico, says Ms Lustig).
Each alignment was check manually in MEGA 5 [ 21], alignments with poor quality or aligned region <100 bp were discarded.
Figure 2A shows the proportion of poor quality ACAs by region in Tanzania based on the artemisinin component.
