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As in CdLS cells, the extent of chromatin decompaction after NIPBL knockdown was greater at the gene-dense regions than at gene poor loci.
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It should be noted that although mtCOI has already proven to be a poor locus for phylogenetic reconstruction for this genus, it has better indicators under both of these tests than the LTRS locus did.
Proximal to the telomeres are the so-called subtelomeric regions, repeat-rich and gene-poor chromosome loci.
Array quality assurance was assessed; poor-performing loci, X- and Y-linked loci, and SNP (single nucleotide polymorphism)–associated loci were removed (Banister et al. 2011), yielding 384,474 loci for 192 infants.
We have identified subtelomerically biased protein families in H. polymorpha, clusters of LTR elements at G + C-poor chromosomal loci in the middle of each of the seven H. polymorpha chromosomes, and established the evolutionary position of H. polymorpha DL1 within a separate yeast clade together with the methylotrophic yeast Pichia pastoris and the non-methylotrophic yeast Dekkera bruxellensis.
The poorest performing locus, CAM-22, failed to amplify in five passerine species (however, all non-passerines amplified; Figure 5).
Although Phusion polymerase is the standard enzyme for Illumina sequencing library amplification, it produces libraries with poor representation of loci with extreme base composition particularly the AT-rich regions [ 4].
Some models also display poor regulation of locus coeruleus neurons and noradrenergic hyperactivity.
Haas et al proposed that these gene-poor repeat-rich loci are dynamic regions of the genome that underpin the evolutionary potential of P. infestans by promoting genome plasticity and enhancing genetic variation of effector genes.
Although the association of these chromosomal loci with poor clinical outcome is novel, the loci have been previously reported altered in OSCC [7], [8], [33].
Other loci provided poor discrimination or were monomorphic.
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