However, focal drift during FRAP could shift plateaus substantially above 1, and cells which moved during FRAP or out of focus debris passing over the cell during imaging resulted in irregular recovery profiles and poor curve-fitting.
Samples with poor melting curve quality were discarded.
As with the overall population, the performance of FeUrea in this patient subgroup was poor (ROC curve AUC 0.58 (0.41 to 0.75)).
Variations in the fluorescence yield upon titration of small molecules with the protein resulted in poor melting curves at low gain while a high gain setting caused signal saturation leading to data loss.
Assays showing poor melting curves were also excluded from analysis.
Primers with low specificity, efficiency and poor melt curves were redesigned.
Unsatisfactorily amplified samples with low RNA concentrations and poor graph curves were also omitted from further processing [ 18].
The discrepancy may be occurring because clinicians and patients are taking into account the personal, physical, social and psychological costs of treatment, and the very poor survival curves associated with current treatment over this age, and decide not to offer or accept RRT.
Consequently, the more prevalent Epi Luminal-B and ERBB2+ breast cancers give rise to poorer survival curves in Epi breast cancer cohorts, suggesting that heterogeneity within a cancer type could mask and perplex the role of EMT.
At sharp curve, poor ambient light can significantly restrict visibility, while visibility is critical for driving in small inter-vehicle spacing.
If the variable takes on smaller values amongst the poor, the concentration curve will lie below the line of equality, and the further below the line of equality, the more concentrated amongst the better off the variable in question is [17].
