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Therefore, these samples with poor coverage depth are not suitable for de novo assembly-based HLA typing.
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Interpreting the TP rates across various intervals (Table 1), it is likely that the lower sensitivities with NX and NG are due to a combination of inadequate coverage depth across what is predominantly coding regions (MIG, Common Target File) and relatively poor coverage outside of coding regions.
Each method used for single-cell genomic DNA amplification has biases to consider and can be affected by poor coverage or uneven sequencing depth, which will result in noisy and inaccurate read counts.
It is therefore difficult to assess a priori which targets will exhibit poor capture and hence lower coverage depth.
The wider gap in coverage depth distribution by Phusion-amplified library indicates uneven and poor coverage of AT-rich regions.
Distributions of coverage depth on chromosomes of the sequencing samples.
A representative coverage depth map is shown in Figure 1.
Agilent and Nimblegen performance were similar at 20× coverage depth, with differences primarily emerging at 30× coverage and above.
The coverage depth was 20.
The other statistics for this assembly were: base coverage = 100%; mismatch = 0%; average coverage depth = 525,995; maximum coverage depth = 9,842.
Universal health coverage in China, the same as in other countries, includes population coverage (coverage breadth), services (benefits) coverage (coverage depth) and financial coverage (coverage height).
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