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This study compared array-based expression patterns of 671 miRNAs using sample pools of ten patients and 18 sex-matched individuals from the different subgroups.
Tan et al. used pools of ten drugs in 384-well plates to study all possible pairs of 1,000 compounds in the minimum number of wells possible in order to find drugs combinations that synergistically prevent HIV replication [72].
To account for biological variation in a spawn we analyzed two pools of ten fresh eggs versus two pools of ten 3-hour aged eggs and the cross comparison of the microarray data show highly correlating replicates (Fig. S1B).
Reverse transcription of RNA extracted from individual samples or pools of ten with random hexamers used the RT system (Promega, United Kingdom) as described previously [44].
Shortly before adding sperm suspension, pools of ten unfertilized eggs were collected from each batch and stored at −80°C for RNA extraction.
Fluorescence activated cell sorting (FACS) was used to purify the GFP+ (green) and GFP− (white) cells from pools of ten neural tubes of mutant and heterozygote Lbx1GFP embryos at E12.5 (Fig. 1B).
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The tournament will be made up of four pools of four teams each.
Two pools of six teams will compete in a round-robin format.
If you wanted to be really clever and cut the length of the tournament by a week you could even recast the tournament into five pools of four, rather than the current four pools of five.
The twelve were organised into two pools of six.
The tournament will consist of two pools of four and two of three.
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