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Two pools of selected 231-BR cells infected with shRNA lentiviruses targeting sh1 sequence were named sh1a and sh1b.
Two pools of selected 231-BR3 cells infected with shRNA lentiviruses targeting sh1 and sh2 sequences were named sh1 and sh2 respectively.
In species with small genomes and less repetitive DNA, the alternative has been to use FISH with pools of selected BAC clones (Lysák et al. 2001).
We ignored the last 25 bp of each read from the VR1-5B strand and the two pools of selected segregants based on the base calling error rate estimated from unique alignments.
Pools of selected segregants of size n were created by recombining the parental strains at a constant recombination rate of 0.37 centimorgans (cM) per kilobase, which is the average value for a yeast chromosome [ 18].
Cells were selected in medium supplemented with puromycin and pools of selected cells were used for further experiments.
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A pool of selected sequences with the highest predicted free energy of binding was synthesized on chromatographic resin, and the resulting adsorbents were tested for IgG binding and resistance to proteases.
A variable number of clones (in the range 100 1,000) were randomly chosen form the pool of selected phage and tested by phage ELISA for their ability to bind the same cell line.
A pool of selected clones was used in this study.
(B ) The ancestor, evolved clone, and pool of selected progeny are sequenced.
In this study, 34 clones were sequenced from the pool of selected single-stranded DNAs.
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