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Pools of control and experimental samples were then combined for intact protein separation.
The coefficients of variation (CV) for estrone and estradiol assays were calculated based on blind pools of control samples included in the analysis.
The results of test samples were compared to the results of two different pools of control DNA samples that were run concurrently.
More than 40 genes from the C-AD library were randomly selected for analysis by qRT-PCR using the pools of control and AD mRNAs as input.
To reveal the molecular events occurring during the drought process, two digital gene expression (DGE) libraries were constructed using RNA from the two pools of control and drought treatment RNA samples and sequenced using Illumina technology.
In brief, for assessment of limit of detection (LoD), linear range, and efficiency of the single PCR assays large pools of control RNA and control DNA from different FFPE tumor blocks were generated and used for the experiments [ 15].
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However, further research with a larger pool of control participants and of patients are needed to be tested beforehand.
We used the pool of control cells as a reference distribution versus the pool of cells exposed to each toxic dose.
For this, we used RSc cells transiently transfected with a pool of siRNAs specific for TSG101, Alix, Vps4B, or CHMP4b as well as a pool of control siRNAs (Con) following HCV infection.
Hybridizations were carried out relative to a common pool of control samples (∼30 control fish) for each tissue, which decreases technical variation as only one reference is utilized while maintaining biological variation of the treatment samples [16].
Subjects were selected from a pool of control subjects as part of a larger fetal MRI study.
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