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Cardiac fibroblasts form one of the largest pools of cells in the heart and contribute to the normal structure and function of the myocardium (Souders et al., 2009).
We generated pools of cells expressing shRNAs that target RAS in a dox-inducible fashion.
As we work with pools of cells, the ability of several PRC1 components to modulate INK4a expression is open to different interpretations.
Both pools of cells were then incubated and tested for peptide bound to the cell surface at 4 and 22 hours.
In our model, a first step is to simulate pools of cells that encode at each position of the input image oriented luminance contrasts which are represented by V1 simple cells in the primary visual cortex [7].
This is accompanied by a clonal continuity between the pools of cells that produce the head and the trunk: indeed, the pool that produces the trunk P-DVCUs is probably entirely derived from cells that have produced head P-DVCUs.
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Once the treatment is stopped, this remaining pool of cells can grow to repopulate the tumour.
For reference, that means if you're trying to modify cells, you've got a pool of cells, and some fraction of those get modified correctly.
Importantly, hierarchical tissue organization, with a few stem cells at the top of the hierarchy, would reduce the pool of cells most susceptible to oncogenic mutations.
Thus, the results of this part of research suggest that CSCs are mainly present in the pool of cells with CD44+ phenotype.
Following acute environmental challenges tissue-resident ILCs expand locally and in this way the pool of cells is renewed, although phenotypic transformation can also occur.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com