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Stranger et al [3] recently showed that pooling total gene expression data from four Hapmap populations assisted in detecting smaller regulatory effects that are shared across populations.
Each individual test sample (Cy5) was hybridized against a differently labeled reference (Cy3), which was constructed by pooling total RNA-isolates from the hypothalamus/pituitary of 49 birds from two different breeds (including the samples of the present study).
by pooling total RNA samples (475 ng) isolated from the dissected nerve tissue of 44 adult worms, synthesizing full length ds-cDNAs using SMART™ (Switching Mechanism at 5' End of RNA Template) technology (Clontech) [48], and normalizing the cDNA pool where known adaptor sequences were incorporated at both ends of cDNA using TRIMMER (Evrogen).
Reference RNA was also included by pooling total RNA from a selection of different cell types.
4049 RA patients who received CZP were included in the safety pooling; total exposure 9277 PY, mean exposure 2.1 years (range 0.04 7.6).
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We isolated total RNAs from frozen liver tissue using CsCl density-gradient centrifugation methods27.We pooled total RNA from the livers of ten wild-type mice and used them as reference in entire microarray experiments.
Similarly, the total pod's weight at Fedis and pooled total pods weight were found to be significantly increased due to combined application of manure, compost and Bradyrhizobium.
Inoculating HUGNR-29 significantly increased pooled total biomass yield by 26.2% compared with uninoculated but this biomass was statistically at par with N-fertilized treatment (Table 3).
Pooled total RNA (1 µg) was used to generate quantitative real-time PCR (qPCR) standard curve for all genes.
Pooled total RNA was further purified using the Qiagen poly(A) RNA isolation kit.
Both pooled total T and free T concentrations decreased significantly following COC use.
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