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Authors' response: Yes, I wish I had thought of that at the time (i.e., pooling reactions using smaller numbers of cycles).
We compared reactions inside and outside the range of venomous snakes using a two-tailed Fisher's exact test, pooling reactions to venomous and non-venomous snakes inside the range of venomous snakes due to the small total sample size.
Moreover, the electron impacts and pooling reactions between the nitrogen metastable states must be considered [47].
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Many excitation processes such as electron collision excitation from the molecular ground state ( {text{N}}_{2} ({text{X}}^{1} varSigma_{g}^ ) ), the first metastable state ( {text{N}}_{2} ({text{A}}^{3} varSigma_{u}^ ) ), pooling reaction and transfer of energy between collisional partners would result in these transitions [27, 28].
Pooled reactions were genotyped using an ABI 3730 DNA Genetic Analyzer and visualised using Genescan.
The second approach, referred to as the pooled reactions consortium analysis, treated the consortium as a single entity.
The pooled reactions consortium analysis method modeled community metabolic potential by treating all enzymatic activities and metabolites as residents of the same physical space, without membrane boundaries.
PCR reactions were cleaned up with AMPure XP (Beckman Coulter Genomics), and pooled reaction yields were quantified using the Qubit fluorometer (Invitrogen).
Since individuals vary with respect to their average response speed, pooling raw reaction times from two individuals might corrupt the link between confidence and reaction time.
By multiplexing PCR and pooling multiplexed reactions together, this method has the potential to score 50 100 SNPs/capillary/run if the sizes of PCR products are arranged at every 5 10 bases from 100 to 600 base range.
Scripts for promoters, bacterial RBSs, and eukaryotic coding regions call BioNetGen to compute all the part or pool reactions and species.
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