Authors' response: Yes, I wish I had thought of that at the time (i.e., pooling reactions using smaller numbers of cycles).
We compared reactions inside and outside the range of venomous snakes using a two-tailed Fisher's exact test, pooling reactions to venomous and non-venomous snakes inside the range of venomous snakes due to the small total sample size.
The intrinsic kinetic rate of WGS (μmol CO g−1 s−1) was correlated with the concentration of the active "carbon-containing" (C-pool) and "hydrogen-containing" (H-pool) reaction intermediates formed within a reactive zone (Δx, Å) around each Pt nanoparticle (1.2 1.5 nm), parameters that were estimated via SSITKA and non steady-state transient isotopic and titration with water operando experiments.
PCR reactions were cleaned up with AMPure XP (Beckman Coulter Genomics), and pooled reaction yields were quantified using the Qubit fluorometer (Invitrogen).
This flux is (A18) The principal input to the pool is due to sucrose synthesis from glucose and fructose, reaction (2), with [eqn (A3)] (A19) A second input is from the kestose pool, reaction (4), given by (A20) This output from the kestose pool is calculated in eqn (A25).
Pooled reactions were genotyped using an ABI 3730 DNA Genetic Analyzer and visualised using Genescan.
The second approach, referred to as the pooled reactions consortium analysis, treated the consortium as a single entity.
The pooled reactions consortium analysis method modeled community metabolic potential by treating all enzymatic activities and metabolites as residents of the same physical space, without membrane boundaries.
Scripts for promoters, bacterial RBSs, and eukaryotic coding regions call BioNetGen to compute all the part or pool reactions and species.
Since individuals vary with respect to their average response speed, pooling raw reaction times from two individuals might corrupt the link between confidence and reaction time.
