"I slipped on a pool of semen and wrenched my ankle," she confesses.
For each freezing procedure, a pool of semen from at least two males was used.
In contrast, this same multiplex PCR assay detected B. ovis, A. seminis, and H. somni DNA simultaneously in three distinct pools of semen samples from experimentally infected rams.
The multiplex assay was capable of detecting B. ovis, A. seminis, and H. somni DNA simultaneously from genomic bacterial DNA samples and pool of semen samples from experimentally infected rams.
An additional pool of eight semen samples was collected, allowed to liquefy at room temperature for 30 min, and portioned into three fractions, "Pre-SP" (SP obtained from semen prior to freezing at −20°C), "Post-SP" (SP obtained from semen after freezing) and whole semen.
In Phase 1, sperm motility was evaluated using a pool of three four semen samples per treatment.
Kurt Cobain, recounting a Neal Pollack dream in his diary, writes: "He stands in a pool of his own semen and vomits bile.
Would you have bathed in a kiddie pool of urine, vomit, semen, fecal matter and who knows what else if your prospective frat or sorority demanded you do so in order to join?
Semen analysis revealed a significant reduction of semen volume and an increase in semen pH in men with OP metabolites.
To increase the probability of SBV infection of injected cattle, 5 straws of semen (≈220 µL each) from 1 batch from an individual bull were pooled and diluted in minimal essential medium with antibiotics to 4 mL.
