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When budgets are tight, some states pool samples to save dollars, but pooling of samples potentially dilutes the levels of substances.
However, the apparent lack of regionality (with respect to these mineralogical and elemental analyses) may be an artifact of sample size (as other geochemical parameters are known to be highly, regionally variable in the Marcellus play [5, 7]) or may arise from the pooling of samples from unique strata.
Moreover, pooling of samples reduces the influence of individual changes in protein composition and favors those proteins present in all the individual samples that may serve as more robust biomarker candidates.
In combination with the indexing and pooling of samples, targeted genomic enrichment allows for the sequencing of a smaller fraction of the genome across a much larger numbers of individuals (reviewed in [1]).
An implied corollary of this result is that pooling of samples [7], is likely to lead to a loss of information compared to that which would be obtained by evenly dividing the same sequencing effort among libraries prepared from the individual samples.
First of all, with high throughput sequencing and pooling of samples that carry their own recognition sequence the VIDISCA cost per sample is reduced, since selective VIDISCA-PCR, metaphor agarose gel visualization, purification of fragments from gel, TA cloning, colony PCR and subsequent BigDye sequencing can all be omitted.
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Thirty pools of samples were tested by polyacrylamide gel electrophoresis (PAGE) yielding a 30% (9/30) positivity.
This paper proposes a new method to divide a pool of samples into calibration and validation subsets for multivariate modelling.
The subagging member models are generated by subsampling the pool of samples available for modelling and then forming new calibration sets.
At least three pools of samples were collected.
Tissues of four individuals per population were randomly sampled, combined, and three pools of samples were formed and separately analyzed.
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