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If a more even representation of all samples is desired, a normalization step can be included before pooling (note that this adds about $1−3 per sample, depending on the quantitative PCR platform and reagents needed for quantifying the libraries before pooling).
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This number of individuals/pool was selected since we have extensively validated use of pools of this size [13] [17], [37], [38] with respect to statistical power as well as the advantages and disadvantages of pooling noted above.
The jurors, armed with a pile of sticky notes and colored markers, meticulously pooled their notes together to reconstruct the timeline around Mr. Rajaratnam's trades in stocks like Clearwire and Intel.
Treble-knotting its tie straps above my buttocks I slipped into the main pool, noting a dozen stout and swarthy forms spaced neatly around its circumference, like digits on a clock face.
"Glance at the men," the pooler noted, "and you know that it has been a long time since Richard Burton" — the nineteenth-century British explorer — "observed that he had never seen a fat man in the desert".
Our future president probably also discovered, as I did, that wonderful community known as the law school study group, where overworked students divide responsibility for portions of a syllabus and then pool their notes and interpretations.
"It started with money NIH had pooled," she notes, "but Congress really liked it, and [the Common Fund] has grown".
The fecundity data across four replicate populations were pooled after noting that there was no main effect of the replicate populations.
As already noted, pooling studies spanning different regions of the x-axis of Figure 1 would inevitably result in diminution of apparent effect size.
Statistical heterogeneity was noted upon pooling positive likelihood ratios when those with predisposition to disease were included (p < 0.001).
Of note, pooled analyses of individual-level data are usually superior to meta-analyses of published estimates, because the former can standardize exposure and covariate definitions, exclusion criteria, and methods for statistical adjustments, and easily facilitate crucial stratified analyses.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com