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All dialysate during the simulated HD session was collected pooled, volume measured, and then sampled (15 ml) for iron sucrose.
CSF samples 30-400 μL from human subjects; pooled volume of 15 μL from 3 mice), or plasma samples (0.5 μL) were denatured at 50°C for 15 min or alternatively at 98°C for 5 min for other analyses.
Dialysate was sampled (5 ml) at 0, 60, 120, 180, and 240 min. All dialysate during the simulated HD session was collected pooled, volume measured, and then sampled (5 ml) for iron sucrose or iron dextran concentration.
These data were used to fit a digital elevation model and a curve for estimating pooled volume given the distance from the playa edge to the intersection of the pool edge with the reference transect.
After each 30 min collection, the pooled volume in the first four bottles and separately, in the fifth bottle, were weighed and sampled.
The pooled volume for each cycle condition will be 300 μl and there will be five tubes in total. 5. Add 10 μl of 5 M NaCl and 1 μl of 20 mg/ml of Proteinase K to each tube.
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In previous studies, the occurrence of Daphnia in rock pools has been correlated to pool volume, organic carbon content of the water, pH or salinity [16], [18].
Separately, the dark and light samples for each rat were then pooled and volume measured.
Inter-session pooled MCP volume SD was 0.1 ml (CV, 1.3%) and SDI SD was 0.1 mm (CV, 1.1%).
Blood from two mice was pooled (total volume of approximately 1.4 mL); plasma and PBLs were then isolated using Ficoll Paque gradient (GE Healthcare Europe, Diegem, Belgium).
After adaptor ligation, ligase was heat inactivated at 65°C for 10 minutes, and samples were pooled by volume into a single tube.
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