Sentence examples for pooled template from inspiring English sources

In this second, overlapping PCR reaction, the overlapping PCR primer pairs of all the exons and the outmost primers (0.1 µM each) were pooled and used in a 25 µl reaction mixture containing 2 µl of the pooled template and the other components (see above).

Amplification, CJE digestion, electrophoresis, and sequencing were performed as previously described [ 20, 35] except that 0.15 ng/μl of pooled template was used.

By sequencing pooled template DNA, we have identified nearly 7000 SNPs in Anopheles gambiae, primarily in or around detoxification-related genes.

The HRM reaction mix contained 10 μl 2XHRM mix (BioRad), 1 μl 5 μM primer mix, 1 μl (10 ng) 6-fish pooled template DNA, and 18 μl H2O.

In a 100-μl reaction, the following were the final concentrations: 1× GoTaq Green Flexi buffer; 0.25× CES; 4.5 mM MgCl2; 200 μM dNTPs; 0.05 U/μl Hot Start Taq; 25 μl of pooled template after Agencourt XP bead cleanup; and 20 μl MiSeq (Fluidigm PN FLD-100-3771) or Ion Torrent (200 nM).

Comparison of SNPs identified from pooled templates with those from individual sequences revealed a very low false positive rate.

With this information, the Construct Design Module can compute a script of pipetting instructions for the Tecan liquid handler to perform all the necessary liquid handling events needed to pool template DNA with amplimers, polymerase, and buffers within individual wells of a 96-well PCR plate.

A paired-end P2 adapter containing T overhangs was ligated to 20 μl of sheared, size-selected, P1-ligated, and pooled DNA template with a specific adapter.

Moreover, the robust sgRNA activity observed when injecting linear dsDNA templates enables other technologies for sgRNA template production such as gene synthesis or oligonucleotide arrays (Bassik et al. 2009; Gilbert et al. 2014), which could be used to create pooled sgRNA template libraries.

Based on this observation, it was hypothesized that if conditions were sufficiently optimized, it should be possible to take the pre-amplification pool template and directly perform the barcoding PCR step, without an intermediate second-stage amplification on the Access Array chip.

Sensitivities were therefore pooled before templates for the α-band of an 11-cis retinal visual pigment [ 72] were fitted to the data using least squares regression.