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Although pyrosequencing pooled specimens is a promising approach for TDR surveillance, there are limitations to extrapolating the results from our study.
The findings presented here are strongly supportive of analyzing pooled specimens for the determination of the prevalence of HIV TDR in HIV PR.
Subsequently, groups of mice were sacrificed 48 or 120 hours post infection and after semi-daily therapy and viral titers were determined from pooled specimens derived from the nasal cavity and lung by plaque assays.
Pooled specimens from spleen-liver and heart tissue were inoculated in embryonated chicken eggs and thereafter in BHK-21 cells (1 ).
Furthermore, NDV was included in the evaluation of swab pooling because it is an important differential of AIV and pooled specimens are often tested for both AIV and NDV.
Very limited data exist on the comparative performance of nucleic acid amplification tests (NATs) for the screening of pooled specimens for acute human immunodeficiency virus (HIV) infection.
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A series of 2-fold dilutions (1 10 to 1 1280) of each groupwise pooled specimen from immunized and control mice were mixed with equal volumes of Wa RV or RRV containing 125 PFU and preincubated at +37 °C for 60 min to ensure that anti-VP6 antibodies would bind to any exposed VP6 in the virion.
We show that pyrosequencing pooled surveillance specimens can cost-competitively detect protease TDR mutations when compared with conventional methods.
Figure 1 presents reverse-phase HPLC profiles showing changes in the composition of venom pooled from specimens from age 8-weeks to adulthood (≥ 36 months).
Since further testing of species limits in these taxa is beyond the scope of the present work, we pooled all specimens belonging to these taxa together under the name T. septentrionalis/ spiniger to recalculate distance measures but indicate a taxonomic issue left to be resolved (Table 1, Additional file 1).
These CSF samples were pooled from specimen collected during our previous study on aSAH that have been published [ 13, 17], in which we showed that CSF samples from aSAH had much higher levels of F4-NPs than that from non-aSAH controls including those with normal pressure hydrocephalus [ 13].
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