The kinetics is influenced by reaction conditions such as the dNTP pool composition and temperature.
The results indicate that the optimal temperature for an equimolar dNTP pool is 72.5∘C and the optimum temperature shifts to lower temperatures when the dNTP pool composition is biased.
The peptide pool composition depends on the type of tissue yet always contains peptides derived from the major chloroplast proteins.
In RP-deficient zebrafish, we found changes in the dNTP pool composition, most notably, a significant increase in dTTP levels.
This finding explained the strong difference found in the soluble amino acid pool composition for this strain [ 8].
Consistently, the bile acid pool composition was similar in Vdr−/− and wildtype BDL mice (Supporting Fig. 3).
Including these structures will provide strong indications about the pool composition, since nearby pools are presumably composed more similarly than distant ones.
Key changes in the microbiome may thus serve as a marker for changes in the bile acid pool composition and vice versa.
Whether this alteration in bile acid pool composition could play a role in abnormal metabolism in diabetes remains speculative, but it is an intriguing possibility.
Pool composition could vary between tissues, and any consequent differences in mitochondrial DNA mutation rate would contribute to tissue-specific age-related mitochondrial change.
