Sentence examples for pooled and subjected from inspiring English sources

Splenocytes from 5 to 6 Cicf/f mice or 3 to 4 Cicf/fCd4-Cre mice were pooled and subjected to cell sorting.

The scFv apoptin contained fractions were pooled and subjected to refolding step via dialysis to remove urea and imidazole.

CTGF containing fractions were pooled and subjected to a second cycle of heparin affinity chromatography.

Subsequently, both in vitro selections were pooled and subjected to an in vivo screening on RMS xenografts.

All the purified interesting proteins from 80 aliquots of MGS were pooled and subjected to further study.

Amplicons from each environmental sample were pooled and subjected to purification using Qiagen's MiniElute PCR purification columns and eluted in 50 µl molecular biology grade water.

For cDNA synthesis the RNA from >10 fetal heart ventricles and ≥3 postnatal heart ventricles was pooled and subjected to reverse transcription using M-MLV reverse transcriptase (SIGMA).

RNA was isolated from six biological replicates from each group using Qiagen RNeasy MiniKit (Qiagen), pooled, and subjected to first-strand cDNA synthesis using Reverse Transcription System (Promega) according to the manufacturer's protocol.

Fractions exhibiting the highest levels of unlinkase activity from sucrose gradients were pooled and subjected to anion-exchange chromatography (DEAE Sepharose FF, GE Healthcare), and proteins were eluted using increasing concentrations of NaCl in unlinkase buffer.

Fractions of the gradient were collected and the radioactivity of each fraction was determined by scintillation counting; peak fractions were pooled and subjected to two rounds of phenol/chloroform extraction and ethanol precipitation.

RNA was isolated from six biological replicates from each group using Qiagen RNeasy MiniKit (Qiagen), pooled, and subjected to first-strand cDNA synthesis using Reverse Transcription System (Promega, A3500) according to the manufacturer's instructions.