Sentence examples for pooled and exchanged from inspiring English sources

Highest purity fractions were pooled and exchanged into binding buffer for EMSA experiments.

4-MUCT-active fractions were pooled and exchanged to buffer A using Vivaspin 3 kDa columns (GE Healthcare).

Fab peak elution fractions were pooled and exchanged into ddH2O via five dilute-concentrate cycles and brought to final concentrations of 15.0 mg/mL.

Appreciable amounts of soluble protein were detected in both cell lysate solutions (from first and second pelleting), and thus the lysate was pooled and exchanged with dialysis into 50 mM potassium phosphate buffer, pH 7.5, overnight at 4 °C.

Fractions containing ComFB were pooled and exchanged into a low-salt buffer [12.5 mM Hepes (pH 8.0), 75 mM NaCl, 2 mM BME, 5% glycerol] using a series of three 5-ml HiTrap Desalt (GE Healthcare Life Sciences) columns.

Eluted fractions were pooled and buffer exchanged into 20 mM sodium acetate, pH 5.6, and loaded onto a Mono S 10/100 GL column (GE Healthcare).

Peak fractions were pooled and buffer exchanged into 2 ml Protein L binding buffer (100 mM sodium phosphate buffer pH 7.2, 150 mM NaCl) with a Amicon 30000 MWCO concentrator (Millipore, United States) and then bound to 400 µl Protein L agarose bead 50% slurry (Pierce, United States) overnight at 4°C.

When Silver finished and was told her time, she shimmied out of the pool and exchanged high-fives with Teri McKeever, her coach at California.

Bound protein was eluted with a linear salt gradient from 0 to 1 M NaCl in buffer D. The BiP-enriched eluate fractions were pooled and buffer was exchanged against HKM using a CentriPure P25 desalting column (emp BIOTECH).

Pure and active fractions were pooled and buffer was exchanged using Vivaspin columns (30 kDa NMWL, Millipore, Germany) in order to transfer the protein into a storage buffer (20 mM Tris Cl, 25 mM NaCl, pH 7.5) and to remove imidazole.

In brief, His-C2I was eluted with PBS containing 50 and 100 mM imidazole, fractions were pooled and buffer exchange and concentrating of protein was achieved with VivaSpin columns (Sartorius, Göttingen, Germany) according to the manufacturer's instructions.