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Fractions were analyzed by SDS-PAGE, pooled, and concentrated, and the concentration estimated spectrophotometrically (280 nm).
Fractions that were positive for agarase activity were pooled and concentrated using an ultrafiltration concentrator.
The culture supernatants containing packaged lentivirus were pooled and concentrated using a Lenti-X concentrator (Clontech).
Peak fractions were pooled and concentrated to 7.0 mg/ml for crystallization.
Fractions containing Cas9 were pooled and concentrated to 10 20 mg ml−1 and aliquots flash frozen in liquid nitrogen.
Fraction D2 was pooled and concentrated.
Fractions from the single major peak were pooled and concentrated to 6 mg/mL for crystallization.
Fractions (25 to 27) with protease activity (Figure 2) were pooled and concentrated for characterization studies.
Fractions corresponding to the protein monomer were pooled and concentrated.
Eluate fractions containing the Prod1 monomer were pooled and concentrated.
Elution was performed with the same buffer, pooled and concentrated.
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