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I314V was the most frequent mutation, and if its frequency is compared to frequency of avian N2, where the same mutation was also prevalent (Table 3), then this NA variant might have originated from avian NA, where it was likely a part of a normal gene pool variation for the N2 subtype.
Again between pool variation was examined in LIMMA.
Between pool variation was also examined in LIMMA.
Additionally, a random effects model was used to pool variation between studies [ 33] in the current meta-regression model.
As our experimental design established a gradient of species richness that increased within a finite species pool, variation across treatments was likely to be unequal [ 40], and this was confirmed by plots of the model residuals.
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This is an important oversight given the intensive land-conversion generated by linear infrastructure development, as well as the relevance given to carbon pool variations associated with land use and land-use changes by national inventories of GHG emissions and global reports.
Quality control evaluations come chiefly from assessment of the array-to-array variation in hybridization intensities noted for replicate experiments, as well as assessments of pool-to-pool variation, as noted here.
This induces large between-pool variation which, together with other sources of experimental error, creates over-dispersed data.
Briefly, within-pool variation is that observed between two arrays used to allelotype the same DNA pool (i.e. replicate arrays), and is an estimate of array variance.
Between-pool variation is that observed between two arrays used to allelotype two different DNA pools, and is an estimate of pooling variance.
To estimate the within-pool variation of theta, replicates of the same DNA pool were run on different arrays (array replicates, as in Earp et al. [ 23]) (Table 1).
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