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The Gaussian Naïve Bayes (GNB) pooled variance classifier was used [13].

The hybrid models combining pooled variance t-tests and Relief algorithm also identified a 15-gene signature (Table S1).

The extra-binomial model still assumes the allele counts obey a binomial distribution within pools and also takes the between-pool variance into consideration.

Pooled variance estimates were used.

Hence, parametric t-tests are always pooled variance t-tests.

Pooling variance is the sum of many sources of variation, including in particular, array variance and pool construction variance.

Var epooling-2) estimates followed the same pattern, larger estimates of pooling Var epooling-2ool-construction variancestimatesot shown).

On average we attribute 30% of pooling variance to pool construction when using Illumina arrays.

Estimates of array variance and pooling variance are used to calculate pool construction variance by subtraction [ 17].

Pool-construction variance is then calculated by subtracting the array variance (Type A) from the pooling variance (Type B).

Var econstruction) accounted for between 20-40% of pooling variance across 27 pools in normalized data.

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