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As for an output pool, the input pool was diluted 500-fold and cultured further for 2 days.
We note that our results would be conservative under a hypothetical scenario in which only one of the sexes contributed to expression differences between ecotypes, by averaging in each pool the input due to males and females.
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The result of qChIP-PCR revealed significant enrichment of all the CArG boxes in the IPed DNA pools relative to the total chromatin DNA pools (the input DNA) except INV (0.7-fold) and EXP1-a (the enrichment of which failed to be monitored due to unstable PCR amplification).
For the optimization experiments, ∼10% of the selected pool and the input sample were reverse-transcribed in a separate reaction for GFPapt quantitation.
Pooling the inputs from six photoreceptors for each sampling point in a visual scene [20] boosted the signal-to-noise ratio in LMCs, broadening its frequency range over that of photoreceptors [3], [17], [21].
Figure 7A shows the PoolingPlanner input panel for the case pool; Figure 7B the input panel for the control pool.
The relative representation of each mutant after in vivo challenge was determined by calculating the ratio present in the output pool compared to the ratio present in the input pool (reads at each insertion/total reads in output divided by reads at each insertion/total reads in input).
Such fluxes may exist and could be linked to outputs from a litter pool or to the inputs to that pool; they are omitted so that we retain the simplicity of a single respiratory output flux.
Approximately 104 CFU of each input pool were used to infect both ears of two chinchillas by injecting 100 µl of the input pool suspension across the tympanic membrane using 27-gauge insulin syringes.
The eDNA pool gains input from the population due to natural cell death and also undergoes spontaneous decay.
The inoculum was plated on 7H10 agar media for colony quantification as well as to study the input pool.
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