Sentence examples for pool sequencing from inspiring English sources

These regions were amplified by PCR, and the PCR products were treated with exonuclease 1 and shrimp alkaline phosphatase to remove unused PCR primers and dNTPs and then sequenced directly using BigDye reagents (ABI, Carlsbad, CA) on an ABI 3730 capillary sequencer (provided by the Gene Pool Sequencing Facility, University of Edinburgh) in both directions.

Using a transposon mutant pool sequencing (Tn-Seq) approach, we identified three genes, sanA, dacA, and yhdP, that are necessary for RpoS-dependent SDS resistance in carbon-limited stationary phase.

Pool sequencing was used to help identify aptamer candidates by generating large quantities of sequences.

The analysis of HLA-I specificities have classically entailed the identification of peptide binding motifs (characterized primarily by the requirement for a few properly spaced and essential primary anchor residues) through pool sequencing of MHC eluted peptides [6] and/or the generation of a representative set of peptide binding data [7], [8].

Additional file 1: BAC pool sequencing and alignment statistics.

Once BAC pool sequencing is done, there are no additional expenses for in silico anchoring using different parameters.

It enabled her to reshoot three of the four destroyed scenes and to use digital technology to restore the pool sequence.

It's an opportunity squandered because Lawrence and Pratt have a spicy chemistry, and the effects, particularly a zero-gravity swimming pool sequence, are impressive.

Then there's the swimming pool sequence.

The array also featured library, R1 R3 pool sequences and R4 aptamer sequences with different linkers.

Aligned reads from all the samples were further processed to pool sequences representing possible isoforms.