Exact(2)
Regarding the latter, clustered pool references, signal intensity, and allelic intensity (B allele frequency) were used to identify CNV [ 14- 16].
Copy number variants were detected using three different platforms: sequence read-depth analysis; CGH arrays by signal intensity variation by comparing two samples; and SNP arrays by clustered pool references, signal intensity, and allelic intensity [ 29, 60, 61].
Similar(58)
This FPE colon RNA pool reference sample was then included during reverse transcription with every patient sample batch and used to monitor process stability throughout the study.
Readings for all time points from such mice were removed from the experiments here for both presentation of data and statistical analyses, and used instead to provide a mixed pool reference to support findings from the primary longitudinal experiment.
Standardized operating procedures and pooled reference samples were created to allow cross-project comparisons and assessment of batch effects.
We found, as reported by Peiffer and co-workers [4], that using a local normal tissue in this manner gave a much cleaner baseline signal compared to using the Illumina pooled reference.
This pooled reference comprised equal quantitites of aRNA from all 20 biological replicate pools.
The pooled reference allowed technical variation to be observed, see Fig. 1b for proteomic workflow.
A dual-labelled experimental design was employed, with each sample being competitively hybridised against a common pooled-reference.
Fold change differences between brain reference RNA and pooled reference RNA were used to assess the reliability of gene expression profiles at different levels of RNA fragmentation.
A dual-label experimental design was employed for the microarray hybridisations with Cy3-labelled test samples competitively hybridised to a common Cy5-labelled pooled-reference per array.
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