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As we have little manipulative capability to guide the cellular DNA repair process to achieve a given outcome, current best practice is to screen through the pool of targeted cells or organisms for a specific modification at the targeted locus.
To understand how αPS2 is retained in the perinuclear region of muscle cells, we hypothesized that αPS2 could normally be locally translated from a pool of targeted mRNAs around the nucleus followed by active transport to its final destination, as it is the case for Gurken in the Drosophila oocyte (Herpers and Rabouille, 2004).
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This targeted cleavage results in an amplified piRNA pool capable of targeting both sense and antisense transposon transcripts.
This fusion framework also allows for creating a dynamic template pool of target features which gets updated over time.
Bigger market share means a bigger pool of targets making it worth the criminals' time to build the malware.
Zafu.com has an innovation that narrows the search for a pair of women's jeans while broadening the pool of targets.
The transformed bacteria were grown in two 500 ml LBAMP media overnight before extraction of the plasmid libraries that contained a pool of target sequences (Plasmid Plus Mega kit, Qiagen).
One downside of retargeting is the potential to oversaturate the pool of targets.
The internet gives offenders access to a global pool of targets.
The faces for each array were randomly selected (the target from the pre-determined pool of targets and the fillers from the pre-determined pool of fillers) and the position of the target was randomly determined for each test trial.
However, by using a related model organism as a proxy for missing functional genomic data and applying multiple layers of subtractive filters based on comparative sequence analysis, we can pre-validate a pool of targets to facilitate their entry into drug discovery programs.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com