P. argentipes sand flies were captured in all 19 wards of the town; in 13 out of 121 pools of sand flies captured, the presence of L. donovani infection could be confirmed.
Every pool sample of sand fly DNA showed the expected 220 bp band due to amplification of the IVS6 region (data not shown).
He stamps on it, conjuring up a pool of viscous sand that bubbles around his feet, drawing them inexorably downwards.
The minimum infection rates (MIR) by Leishmania in the captured phlebotomine sand flies were calculated as the ratio of the number of positive pools of each sand fly species and the number of specimens in that pool, multiplied by 100 [ 17].
Pools of 10 sand fly salivary gland pairs were collected in 50 μL of phosphate-buffered saline (PBS) on ice and individually pierced to release their saliva.
Genomic DNA analysis of 136 species-specific pools of female sand flies followed by molecular genotyping showed the presence of Leishmania infantum DNA in two pools of Lu. longipalpis.
To generate a comprehensive adult reference transcriptome of P. perniciosus and to investigate sex-biased gene expression levels at adult stage, we produced six Illumina RNA-seq libraries, with an average insert size of 130 bp, from three pools of adult sand flies for each sex.
The first library was constructed from a pool of midguts from sand flies allowed to feed on sucrose solution (sugar fed).
On the upside, this is the long-awaited UK theatre debut of the legendary French director Patrice Chéreau: a production stunningly staged on a strip of sand pooling with dark water.
These conditions included one library combining the midguts from sand flies allowed to feed on a sucrose solution (SF), a pool of midgut tissue from sand flies fully engorged from an artificial blood meal 1, 2, and 3 days post blood meal ingestion (BF), and a pool of midguts from gravid sand flies 5, 6, and 7 days post blood meal digestion (PBMD).
