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iii) Under such conditions, the ee value of the 1,4-addition product, produced by an asymmetric source of Nu− should decrease over time (due to competition with an increasingly populated pool of promoter anions).
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As shown in Figure 2, there was a highly significant correlation between H3K4me3 and AcH3 in three cell populations, while the H3K27me3 mark was present on a distinct pool of gene promoters.
This procedure involved an iterative pair-wise motif sampling process between the promoter of the atoDAEB operon, which was used as the initial qualified sequence, and a pool of E. coli promoters (initial promoter set).
We randomly select a similar number of promoters as in the original data set from the pool of all human promoter regions and compute a new score for each TF as before (Fig. 2c).
A study using a pool of 6500 synthetic promoters, each upstream of an identical YFP reporter, compared the relative expression conferred by different combinations and positions of yeast TF binding sites and other sequence features (Sharon et al. 2012).
After confirming the effect of the phosphomimetic mutation S76D on the pool of DegU∼P using the comK promoter, activated by unphosphorylated DegU, we set out to confirm this finding from the opposite angle.
In these studies, a full-length cDNA sequence is positioned between two identical promoters such that independent transcription from each promoter produces a pool of sense and antisense RNAs capable of forming long dsRNA and undergoing processing to the effector siRNAs.
That is, SigB regulon divergence appeared to rely on a process of promoter 're-assignment' of genes from a common gene pool into/out of the SigB regulon.
Hence, this simple strategy of promoter engineering could be an effective way to generate a pool of strong constitutive promoters applicable for heterologous protein expression in B. subtilis in the future.
To differentiate between promoters dependent on the regulator and other promoters, plasmid DNA from a pool of the kanamycin-resistant transformants is transformed into bacteria that do not express the transcriptional regulator.
We used two methods of promoter extraction.
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