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Proteins were isolated separately from tissues dissected from each pool of plants, to produce four paired protein samples from fibres and the non-fibre fraction, where each pair of samples was biologically independent from every other pair.
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While cadging seeds and shoots is somewhat more systematic than relying upon wind currents and bird droppings, my pool of plant talent has been limited by the limits of my friends and family.
In lysimeter and soil extraction samples, nitrate and ammonium were found at the highest concentrations, while results from microdialysis revealed that the pool of plant-available amino acids was contributing most to the total N pool tested.
The ability of heterologous fluorescent actin to incorporate into endogenous pool of plant microfilaments has been under doubt [ 9, 10] but the reason why mammalian actin did not incorporate into plant microfilaments during few published experiments is still unclear [ 10, 11].
Using these simulated phenotypes, we selected two pools of plants representing the extreme 10 or 30% of phenotypic variation.
For each genotype, at least two independent RNA preparations were made from separate plants or pools of plants and electrophoresed in neighboring lanes on RNA gels.
The central idea of BSA is to form DNA pools of plants that differentiate with regard to phenotype.
Recovery of the same FST from both row and column pools of plants was used to associate 528 of the plasmid-rescue insertion sites to individual plants.
However, we could not find a correlation between their expression levels with the flowering phenotype in the F2 pools of plants selected (data not shown).
Table 1 lists a selection of the differentially expressed genes along with the number of biological samples that displayed altered expression when high and low Brix pools of plants were compared (HB vs LB) and when mature and immature internodes were compared (MI vs II).
The particulate phosphorus pool consists of plants and animals and their remains, phosphorus in minerals (e.g., fluorapatite) and phosphate adsorbed onto iron oxyhydroxides on mineral surfaces.
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