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I'm not necessarily saying you have to have monomer-by-monomer synthesis of RNA, but to make RNA you're going to have to make a pool of nucleotides.
As noted above, it is known that D. radiodurans exposed to ionizing radiation produces an intracellular pool of nucleotides which are subsequently converted to nucleosides [22].
The accumulation of Mn is a hallmark of all Deinococcus bacteria [5], [17], [20], and it is established that D. radiodurans exposed to ionizing radiation or UV radiation produces an intracellular pool of nucleotides which are subsequently converted to nucleosides and then exported [22].
GMPS localizes mostly to the cytoplasm, where it maintains the pool of nucleotides.
In S. cerevisiae, HU inhibits the ribonucleotide reductase enzyme, which results in reducing the pool of nucleotides in the cell.
Folate deficiency alters the balance of the pool of nucleotides needed for the synthesis of new DNA molecules, leading to dUMP accumulation.
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To achieve this, HASs draw from a cytosolic pool of nucleotide sugar precursors synthesised by the hexosamine biosynthesis pathway, an alternative glucose metabolism pathway.
Such scaling is not required for the forward polymerases as it was assumed that the probability of a hydrolysis event in this case was averaged over the available pool of nucleotide triphosphates in a time independent manner.
Expression of nucleoside diphosphate kinase Ndk, which is responsible for maintaining a pool of nucleotide triphosphates for the synthesis of DNA and RNA in E. coli [24], was found to be upregulated by about 3-fold in the IrrE-expressing strain in response to salt shock; (iii) Glycerol-degrading enzymes.
SeAMotE is based on the generation of a pool of nucleotide seeds followed by "zero or one occurrence per sequence" (ZOOPs) model testing [ 17] coupled with pattern extension and refinement [ 8].
This gigantic pool of nucleotide variations is constantly updating by 40 100 novel mutations arriving in each person (Kondrashov and Shabalina 2002; Conrad et al. 2011; Li and Durbin 2011).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com