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The quantified libraries were then multiplexed with other libraries, and the pool of libraries was then prepared for sequencing on the Illumina HiSeq sequencing platform using a TruSeq paired-end cluster kit, v.4 and Illumina's cBot instrument to generate a clustered flow cell for sequencing.
The pool of libraries was sequenced on one lane of the HiSeq 2500 (Illumina) at 2x125 bp paired-end sequencing with v4 chemistry.
This type of analysis will return data similar to those in additional file 1, though comparison can also be restricted to specific libraries rather than the pool of libraries evaluated here.
Accurate interval identification is also important in looking for genomic rearrangements such as translocations, and BAIT is able to detect these and assign a frequency of the rearrangement within the pool of libraries, requiring a far lower read depth than conventional split-pair read sequencing [ 27].
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He found a ready pool of library donors in people and companies with matters before the government, many of them loyal Democratic contributors.
Different pools of libraries were set up and sequenced on individual lanes in duplicates to compensate for loading effects.
We apply this method to DNA pools of libraries from several human individuals from which we capture complete mitochondrial (mt) DNAs, a maternally inherited DNA molecule which is extensively studied in population genetics, medicine, forensics, and phylogenetics [8].
Counts of EST tags linked to the same UniGene cluster in pools of libraries were correlated.
Pools of libraries were enriched for 1,977 cancer-related genes using (SureSelect technology, Agilent, Santa Clara, California, US).
Another module is the Digital Gene Expression Displayer (DGED) which distinguishes statistical differences in gene expression between two pools of libraries [ 72].
The low overall coverage obtained for some of the 24 libraries sequenced for this experiment is probably due to uneven pooling of libraries prior to hybridization enrichment.
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