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Previous work has demonstrated that the entire detectable pool of expressed CTA1 is initially delivered to the ER where the signal sequence is removed [26].
This allowed detection of bona fide transcriptionally regulated genes, thus reducing the interference of steady-state mRNA turnover on the pool of expressed mRNA common to conventional microarray analysis.
While the overall pool of expressed transcripts was smaller in VMN than in H (Chi squared test, P < 0.0001), transcript abundances were comparable between tissues in most gene categories.
When comparing the percentage of genes that are sex biased from the total pool of expressed genes in a given tissue, roughly 18% of genes were significantly-biased at the absolute log2 fold-change level >1, though this proportion decreased as the fold-change level increased.
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The phenotypic changes that characterize the aging process are governed by specific alterations in the pools of expressed proteins.
The pool of differentially expressed genes from the expression profiling consisted of target genes that were regulated directly by Bapx1 and Sox9, and indirect target genes whose expression was altered owing to the loss of these two transcription factors affecting their upstream regulators (secondary effectors).
These data are of course preliminary in nature, and require further work to assess the potential consequences of a reduction in GSDMB expression, or an alteration to the pool of transcripts expressed from the GSDMB gene to the function of tissues involved in immune function and inflammation.
Like intertwining cogwheels in a mechanical watch, a pool of periodically expressed genes keeps time in a tiny region of the brain called the suprachiasmatic nucleus.
Essentiality of trans-translation has been proposed to be associated with small genomes or with the necessity to accurately manage a restricted pool of ribosomes expressed by a limited number of rRNA operons [29].
In order to find functional groups of genes over-represented in our dataset, we selected a bigger pool of differentially expressed genes using a less stringent criterion: p-values from t-tests (without FDR correction) less than 0.05, TNoM score equal to 0, and the fold-change in expression greater than 1.5 (on original data scale).
In this fashion a pool of genes expressed with high level of reference concordance was selected.
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CEO of Professional Science Editing for Scientists @ prosciediting.com