Exact(5)
In the current pool of experiments it is possible to observe the scatter in results caused by manufacturing defects and material variations.
Pie charts represent the initial and final proportions of each population category represented in the total pool of experiments.
Standard error bars represent standard errors of the mean (±SEM) calculated from the total pool of experiments; in all cases, paired Student's t-tests were applied to determine significance.
The experiment was repeated at least three times and the means were calculated for the pool of experiments.
From this pool of experiments, the baserate is the a priori probability of any focus to lie in either of the two compared regions/networks (i.e., when randomly drawing a focus that activates superior inferior or superior posterior frontal cortex, what is the probability that it is the posterior one).
Similar(55)
An in-house script, finalList.pl, described previously [47] for applying parsimony (Occam's razor) to generate a non-redundant list of identified proteins from a large pool of independent experiments was adapted to simultaneously calculate average peptide ratios for each protein in each block.
An in-house script, finalList.pl (available here: http://www.chibi.ubc.ca/wp-content/uploads/2013/01/finalList.pl_.txt) for applying parsimony (Occam's razor) to generate a non-redundant list of identified proteins from a large pool of independent experiments was adapted to simultaneously calculate average peptide ratios for each protein in each block.
The data shown in this study represent the results from one RNAseq experiment containing a pool of three independent experiments for each sample.
Thus, these methods require pooling of multiple experiments using different restriction enzymes or primers to come close to saturating the potential insertions.
Because the absolute magnitude of viability data often varies between experiments, we expressed the data as per cent survival (relative to nonirradiated controls) in order to allow the normalisation and pooling of multiple experiments.
Each individual data point was obtained from assessment of 12 images (4 sites/well in triplicate), and normalised as a percentage of the basal controls (set to 100%), to allow pooling of individual experiments.
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