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However, whether the maternal provision of Cdx2 message is superfluous or helpful in enhancing TE specification is not clear and awaits a thorough comparison of the developmental dynamics of embryos with both maternal and zygotic pools of Cdx2 eliminated versus embryos in which only the zygotic pool is removed.
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The pool V is formed such that none of the bit rate targets of the user set G1 would be sacrificed if any one resource block in the pool was removed.
We simulated host-tree extinctions using two different models: (1) "random extinctions", where a randomly-chosen species from the extant species pool was removed at each extinction event, and (2) "rarest-species extinctions", where the least abundant species of the extant species pool was removed at each extinction event.
Replicate spots were averaged, and remaining false barcodes of strains not present in the homozygous or heterozygous deletion pool were removed.
A 125-μL aliquot of the supernatant of each pool was removed and placed in an identically labeled Eppendorf tube for RNA extraction and testing with a real-time reverse transcriptase polymerase chain reaction (RT-PCR) assay (see below).
Twenty-four items from the 37-item pool were removed following the item selection process: nine items were removed due to high item-to-item correlations; five due to floor effects; three due to infrequent activity; one due to gender bias; two due to low factor loadings; three due to unordered response options; and one due to expert's discretion.
After the biggest pools are removed, the so-called "drive on" machines come out.
Note some lifecycle processes, stages and pools were removed from the diagram for clarity.
Repeated application of four of the pools destroyed the membrane seals, and these pools were removed from further analysis (Figure 2A B, grey boxes).
Stably transfected BaF3 and 32Dcl3 cells (WT and K762R mixed-pools) were removed from IL-3-containing media, washed, and then grown in the presence of 10 ng/ml of G-CSF at 37°C overnight.
All fish present in the pools were removed using three different methods: a cylindrical trap (matapi or covo), a seine net (as above) and a hand net.
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