Typically, ∼10 amniocytes were obtained in 100 μl of amniotic fluid per sac (∼0.75 1.0 ml per pooled collection from a single litter).
For competitive growth experiments aliquots of the pooled collection were outgrown at 25° for 210 min before being diluted and shifted to the assay temperature for 20 generations in YPD.
Motifs were obtained by pooling collections from five sources (i.e., Jaspar, UniPROBE, ENCODE, hPDI and TRANSFAC) [ 83- 87], as described in a recent publication [ 65].
Because of obvious advantages (i.e., coverage, mutant identification), most genetic screens are now done on a systematic basis using arrayed or pooled collections of known mutants.
RNA was extracted from 668 mosquito bodies without legs (301 Cx. torrentium, 367 Cx. pipiens) and from 74 pools of mosquitoes pooled by collection trap and week (290 Cs. morsitans); pool sizes ranged from 1 to 19 mosquitoes.
This may take the form of a pool or collection of tickets or their counterfoils from which the winners are extracted.
In Hotel Iris she has retreated into darker territory, a mode closer to The Diving Pool, the collection of novellas that in 2008 was the Anglophone world's stunning introduction to the Japanese writer's work.
The police also guided journalists through the luxurious three-story villa of a Rocinha drug trafficker called Peixe, or Fish, who was arrested last week, showing them his hot tub, swimming pool and collection of imported spirits.
In this paper, an optimized molten pool data collection method called photodiode multi-detector divisional detection was designed to improve the precision of photodiode data during selective laser melting (SLM).
