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Therefore, all clones that contribute to the trunk also contribute to the head (Fig. 2A G, K), indicating that perhaps all of the trunk pool cells are derived from precursor cells common to the head and the trunk.
Pool cells from a particular donor.
There are two approaches: pool virus supernatant of several or all constructs together, or infect cells with the individual constructs and subsequently pool cells.
For immunoblotting assays using the siRNA pool, cells were incubated with 1.25 nM of each siRNA for 24 h; for MTT and apoptosis assays, 0.625, 1.25, 2.5, or 5 nM of each siRNA was incubated with cells for 48 h.
The Src kinase Lyn is unlikely to be involved in C35-induced EMT, because Lyn mRNA levels are reduced by approximately five-fold in C35hi cells, in comparison with both null and C35 transfectant pool cells.
Interestingly, in addition to the cytoplasmic pool, cells also contain a pool of Keap1 that is tethered to the outer mitochondrial membrane forming a ternary complex with Nrf2 and phosphoglycerate mutase 5 (PGamember member of the phosphoglycerate mutase family [ 37].
Similar(53)
This could be through cell-intrinsic alterations in the stem cell pool, cell-extrinsic changes affecting stem cell function, or a combination of both.
On the other hand, genes which promote neuronal differentiation (thus depleting the progenitor pool), cell apoptosis or radial migration attenuate surface expansion and hence gyrification.
To determine if the endoplasmic reticulum accumulated Zn pools, cells were cultured on glass coverslips to ∼80% confluency.
To determine the nature of the mutations observed in the M1 population, we analyzed pooled cells as well as single cell clones.
To confirm these results, pooled cells transfected with VerUTR or the control vector were lysed and probed with anti-PTEN antibody for western blot analysis.
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