SGC-7901 cells (8 × 104 cells/well) were seeded on a 96-well polystyrene plate.
Indeed, one tissue culture polystyrene plate also served as a control surface [7].
The 293T cells (1 × 104 cells/well) were seeded on a 96-well polystyrene plate.
Third, the derivatized copolymer was immobilized by photolithography on a polystyrene plate, and then the surface characterization of AzPhPIA-coated polystyrene plate (PSt) was measured by electron spectroscopy for chemical analysis (ESCA).
The average MTT values for tissue culture polystyrene plate, unanodized and anodized titanium with nanostructure were equal to 0.49, 0.16 and 0.72, respectively (P < 0.05).
The average cell attachment results showed that 9,955 ± 461.18, 3,300 ± 197.98 and 11,359 ± 458.10 EPCs were attached per well of tissue culture polystyrene plate, unanodized and anodized titanium surfaces, respectively (P < 0.05).
SGC-7901 cells with a density of 8 × 104 cells/with were seeded on a 96-well polystyrene plate, andensitywell coftained 100 μL of RPMI-1640 medium supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin solution.
Fig. 4 Morphology of normal human skin fibroblast cells (CRL2522, ATCC) on (a c) the tissue culture polystyrene plate (TCPP), (d f) polyurethane (PU) film (g i) PU film containing 0.01 % wt % MWNT (PU-MWNT01), (j-l) PU film containing 0.04 % wt % MWNT (PU-MWNT04), m o PU film containing 0.08 % wt % MWNT (PU-MWNT08) after cultured for 24, 48 and 72 h.
293 T cells with a density of 1 × 104 cells/well were seeded on a 96-well polystyrene plate, and each well contained 100 μL of DMEM (high glucose) medium supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin solution.
SGC-7901 cells (a seeding density of 1.5 × 105 cells/well) were seeded in Bioptech tissue culture plateseedingtechs Inc., Butler, PA), andensity 105 cells/well of a 6-well polystyrene plate for confocal studies and flow cytometry studies, respectively.
Because direct coating of the native CRP pentamer onto the polystyrene plate surface results in a loss of native CRP antigenicity, the native protein required calcium-dependent capture to a surface immobilized protein carrier (Keyhole Lympet Hemocyanin (KLH) derivitized with native CRP's primary ligand –phopshorylcholine (PC) (i.e., PC-KLH).
