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Less intense, but clearly occurring, is the merge of Sulfolobus poly (ADP-ribose) polymerase with nucleoid.
However, they were defective in two functions that require interaction of polymerase with product RNA.
A polymerase with strand displacement activity first synthesizes semi-amplicons of variable length, which dissociate from the template at high temperature.
Subsequently, in the two rounds of PCR, we utilized Plantinum Pfx DNA polymerase with an error rate of 1/632,900.
As well known, the polymerization is consisted of two processes in PCR system, i.e., the binding of polymerase with primer template junction (PTJ) and extension of the binding.
By regulating the dynamic equilibrium of binding of polymerase with primer temples junction, the PCR process and DNA synthesis could be modulated.
Fast DNA polymerase with a high extension speed was used in this method, and an amplification program was used to rapidly amplify long specific sequences.
A pair of specific primers was designed against the conserved region in the 3D gene that encodes the RNA dependent RNA polymerase with a single conserved TaqMan™ probe.
The feasibility of the system was demonstrated by the detection of IgA and Taq DNA polymerase with high specificity, using two different antiidiotypic affibody pairs.
Our results suggest that DNA polymerase with RT activity and DNA/RNA helicase are useful to increase the sensitivity of cDNA synthesis.
Viable virus was recovered following transfection of mammalian cells, expressing T7 RNA polymerase, with 10 plasmid constructs representing the cloned BTV-1 genome.
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