Sentence examples for polymerase type from inspiring English sources
Cells were transfected with plasmids containing an independently expressed reporter gene and an RNA polymerase type III promoter to constitutively express small stem-loop RNA transcripts corresponding to Oct4 mRNA.
There are two different descriptions of plant mitochondrial transcription that are linked to polymerase type.
The differences in amplification efficiency may be related to both polymerase type and varying buffer conditions among commercial vendors.
Figure 5 shows how numbers of RNA polymerase (the sum of polymerase type I, II, and III) vary from state to state as the cell cycle progresses.
Oliver et al. (6 ) compared sequences of different NB-like and NA1-like strains and showed that strains of the same polymerase genotype presented >88% nt (>95% aa) identity in the polymerase region, while nucleotide sequence identities among strains from distinct polymerase type were <78%88%8% aa) in this region.
However, as previously demonstrated [ 26- 28] the reproducibility of the RAPD analysis is dependent upon a careful standardization of the PCR conditions (especially choice of the primer, DNA and primer concentration, polymerase type or thermal cycler profile are critical factors in producing informative patterns).
Prior to this study we tested a collection of Taq DNA polymerase types, and due to its high fidelity concluded that we should use Phusion Taq DNA polymerase (New England Biolabs UK, Hitchin, UK) for all PCR reactions.
DNA replication errors occur in all DNA polymerases in vitro, but their frequency can vary exponentially among different polymerase types.
Therefore, clustering analysis identified presumably all plastid promoters, which are preferentially transcribed by the two polymerase types.
These ncRNAs are transcribed by at least two RNA polymerase types, and their accumulation is affected by a variety of chloroplast ribonucleases.
This problem should probably be addressed separately for nuclear, plastome and mitochondrial genomes, different taxonomic lineages and different RNA polymerase types.
