Sentence examples for polymerase termination from inspiring English sources

Although in some sporadic cases, additional bands were obtained (marked with small arrowheads in Fig. 3B), sequence analysis showed that they were either non-relevant (sequences unrelated to miR-375) or shorter species, most likely resulting from premature polymerase termination caused by high GC content, or stable secondary structure of the pri-miR-375.

The function appears to be the dephosphorylation of serine residues within the conserved heptad repeat in the C-terminal, which is required to reactivate the polymerase after termination of transcription.

Here, Proudfoot, Dye, and colleagues report a genome-wide terminator analysis in which they identify 78 cotranscriptional cleavage (CoTC -type terminator elements and analyze different, possibly overlapping, RNA polymerase II termination pathways operating at human gene loCoTC -type

This cassette comprises inverted repeat sequences (CX3 or CX5) against the target gene (OsCKX2), a spacer fragment (loop) and RNA polymerase III termination sites.

The sense strand comprises a random 29-nt sequence ("N29"), a non-complementary loop sequence, the reverse complement ("n29") of the random 29-nt sequence, and a polymerase III termination sequence.

Common primer 2 is a minus strand oligonucleotide encoding a terminal AttB2 site, an optional FRT site, 13 nucleotides of the human miR-30a lower stem, and a RNA polymerase III termination signal (TTTTTT).

Depletion of Rai1 or Grc3 impairs Rat1-dependent degradation of the 3′ trailing transcript in RNA polymerase I termination (El Hage et al, 2008; Kawauchi et al, 2008; Braglia et al, 2010).

NusA has been shown to stimulate pausing of the elongating RNA polymerase, facilitating transcription termination at rho-independent terminators [ 45, 46].

Whereas, an early formulation of the torpedo model suggests that a 5' to 3' exonuclease attacks the transcript at the poly(A) cleavage point and degrades the nascent transcript in pursuit of the RNA polymerase, leading to termination [9].

Each shRNA sequence comprises a selected siRNA sequence targeting the murine GFAP mRNA (NCBI Accession Number NM_010277) or murine vimentin mRNA (NCBI Acession Number NM_011701) in both sense and antisense orientations, separated by a 9 nucleotides loop (5'-TTCAAGAGA-3') and followed by five thymidine residues as an RNA polymerase III transcription termination signal.

Likewise, we expect our conclusions to be robust to increasing molecular details and more realistic modelling of each subprocess (RNA polymerase, initiation, elongation, termination, ribosomes…).