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PCR was performed using the Tks Gflex DNA Polymerase system (Takara, Shiga, Japan).
The cDNA was amplified by PCR using Accuprime Taq DNA polymerase system (Invitrogene).
The cDNA was then amplified by PCR using the AccuPrime Taq DNA polymerase system (Invitrogen).
A region of the MDM2 (AF_527840) promoter was amplified using the DyNazyme EXT polymerase system (FINNZYMES) according to the manufacturer's instructions with primers MDM2PF-CGGGAGTTCAGGGTAAAGGT and MDM2PR-AGCAAGTCGGTGCTTACCTG.
Primers for GFP, Oct-4, β-Casein, or GAPDH were used for PCR amplification using the AccuPrime Taq DNA Polymerase System (Invitrogen).
The genomic fragments of the Oscarella and Taeniopygia tetraspanin genes were amplified by PCR using the AccuPrime Taq DNA polymerase System (Invitrogene, California, U.S.A).
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For PCR reactions, the HotStarTaq (QIAGEN) and FastStart High Fidelity (Roche) DNA polymerase systems were used according to manufacturer's instructions.
Thus, ImuB-C appears to have functional connections with several major mutagenic DNA polymerase systems (network in Figure 1).
Proton inventory on the RNAP reaction in both wild-type and trigger-loop deletion mutants of RNAP, like those performed for other polymerase systems by the Cameron group [ 8], will be important for advancing our understanding of how multisubunit RNAPs may be distinct from other polymerases.
Two mitochondrial loci were amplified by using the Sigma Taq-Polymerase system: partial NADH dehydrogenase subunit 2 (ND2, 830 bp length) and a combined fragment comprising partial 12S rRNA, tRNA-Val, and partial 16S rRNA 12S-16S6S fragment"; ca. 1,275 bp length).
The Cys-mutated rmCRP gene was cloned into the T7 RNA polymerase expression system under the lacUV5 promoter (Tabor and Richardson 1985), and transformed into E. coli BLR DE3).
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