Exact(1)
Application of ultrafiltration with Millipore filters (YM100) was reported to reduce Taq polymerase sensitivity in one case [12], but not in another [13].
Similar(59)
Reagents can have a significant effect on assay reproducibility [ 27] due to some parameters such as different polymerases sensitivity [ 28], primer binding efficiency and the concentration of Mg2+ [ 29].
The search terms used were: tuberculosis, Mycobacterium tuberculosis, nucleic acid amplification techniques, polymerase chain reaction, sensitivity and specificity, and accuracy.
Steady-state analyses of the impact of silencing on T7 RNA polymerase obscured the sensitivity to Sir-based silencing that was revealed in kinetic experiments.
Preliminary experiments with a range of commercially available Taq DNA polymerases had shown that these two polymerases had the highest sensitivity and specificity when used with cDNA derived from frozen breast tumour specimens.
The sensitivity of polymerase chain reaction (PCR) in cell detection was negatively affected by some magnetic carriers and compounds used in their preparation.
The utility and increased sensitivity of polymerase chain reaction (PCR) tests have been reported; however, their use in US hospital laboratories is not well characterized.
Background: For many diagnostic applications, the specificity and sensitivity of polymerase chain reaction (PCR) is markedly enhanced by applying two rounds of PCR with nested or semi-nested pairs of primers.
The sensitivity of polymerase chain reaction (PCR) testing can be limited by the copy number of the pathogen in question.
This low-cost assay is readily available throughout Latin America [ 27] but has a lower sensitivity than polymerase chain reaction (PCR) [ 28].
Previous studies have shown that φ29-based amplification in the presence of a second strand-displacing DNA polymerase improves DNA microarray sensitivity relative to multiplex PCR amplification or amplification with φ29 DNA polymerase alone [ 10].
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