Right now the polymerase in PacBio's prototypes is assembling DNA at a speed of up to 10 bases a second — far slower than it would in a naturally dividing cell.
This phenomenon was validated with rTaq and power Taq polymerase in our study.
The mismatched primers cannot be extended by Bst DNA polymerase in the absence of Hg2+.
T7 RNA polymerase was superior to Escherichia coli RNA polymerase in the specific transcription of the MG RNA aptamer.
The library was then PCR amplified using Phusion DNA polymerase in HF buffer for 14 cycles and quantified using the Agilent BioAnalyzer (Santa Clara, CA).
PCNA can form a homotrimeric ring-shaped structure that encircles DNA and acts as a clamp to assist DNA polymerase in mediating DNA replication (Moldovan et al., 2007).
Point mutations, insertions, or deletions are introduced by incorporating DNA primers containing the desired modification with a DNA polymerase in an amplification reaction.
These antibodies will be useful in experiments designed to describe more fully the role of the viral DNA polymerase in DNA replication of vaccinia virus.
When the linearized vector is treated with T4 DNA polymerase, in the presence of the absent base, long single-stranded overhangs are generated that are suitable for cloning.
Functional analysis showed that dsVHL-821 induced apoptosis by increasing p53, decreasing Bcl-xL, activating caspase 3/7 and poly-ADP-ribose polymerase in a dose-dependent manner.
