Sentence examples for polymerase conducted from inspiring English sources

When presented with a primed (6 S -HNE-1 S -HNE-1, N-dGuo:dCyd pair, the polymeraS -HNE-1cted full-length primer extension.

The Dpo4 polymerase conducts error-prone replication past the AFB1 FAPY adduct, including misinsertion of dATP, consistent with G → T transversions.

Human polymerase η conducted error-prone replication past 1, N-εdG, preferentially incorporating dGTP, irrespective of the identity of the base 5′ to 1, N-εdG in the template (71).

PCR amplification by Taq polymerase was conducted using a program of 94°C for 2 minutes, 55°C for 1 minute, and 72°C for 2 minute for OsCam1-1; OsCam3-2; OsCand; and OsCam3 and a program of 94°C for 2 minutes, 58°C for 1 minute, and 72°C for 2 minute for OsCam1-3.

The binding of SMRT bell templates to polymerases was conducted using the DNA/Polymerase Binding Kit P5 and v2 primers.

In vitro DNA polymerase reactions were conducted using DNA polymerase β (Pol β) to derive the frequency of unit-based indel errors (polymerase error frequency [Pol EF]), which presumably result from slippage, for TRs at varying motif size, sequence and repeat number (supplementary fig. S5, Supplementary Material online).

Chromatin immunoprecipitation for RNA polymerase II was conducted according to the following procedure modified from the published method (Yu et al., 2015).

Polymerase chain reaction conducted on the fluid aspirate from the enucleated eyeball was positive for two TB specific primers (IS6110 and MPT64) (c) Fig. 5 Fundus photograph of the right eye at the final follow-up visit showed resolution of optic disc edema and decrease in vitritis with multiple grayish lesions in the periphery in various stages of healing (a).

DNA end-repair with T4 DNA polymerase (NEB) was conducted following the manufacturer's protocol.

The conditions for the PCR amplification were as follows: polymerase activation was conducted at 95°C for 30 s; followed by 40 cycles at 95°C for 5 s, 60°C for 31 s.

We conducted polymerase chain reaction (PCR -based assays for PCR -basedeassaysmors and analyzed publically available array-comparative genomic hybridization data to map a common copy-number breakpoint in ERBB2-ampliford primary breast tumors.