Exact(6)
Overall a picture emerges of influenza polymerase being a highly complex, flexible and dynamic machine.
A touchdown PCR was used with an initial denaturation at 94°C for 2 10 min depending on the manufacturer's recommendation for the Taq DNA polymerase being used.
Any interaction between the two fusion proteins results in RNA polymerase being recruited to the promoter, and can therefore be detected by monitoring lacZ expression.
Perhaps RCA is a mechanism that evolved later, after the emergence of an efficient RNA polymerase (being it protein enzyme or ribozyme), and it is likely a strategy of parasites.
Our finding that Mn2+ increased the rate of null mutation (Table 1) is consistent with the error rate of Taq polymerase being dependent on the concentration of the cation.
The idea of another RNA polymerase being involved in generation of TERRA would be consistent with recent data in which several subunits of RNAPI and RNAPIII (RPABC1, RPAC1, RPA49, RPA2 and RPA1) were found enriched in formaldehyde-crosslinked and partially purified human telomere fractions (Dejardin and Kingston, 2009).
Similar(54)
The enzyme, known as Taq DNA polymerase, is used in the polymerase chain reaction, a technique to amplify minuscule amounts of DNA for use in medical diagnosis and DNA fingerprinting.
DNA polymerase was Phusion (NEB, Hitchin, UK).
Taq DNA polymerase was purchased from CW Bio (Beijing, China).
The Pfu DNA polymerase was purchased from Tiangen (Beijing, China).
The fidelity of the PCR polymerase was also evaluated.
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