Sentence examples for polymerase amplified from inspiring English sources

Quantitative polymerase chain reaction (qPCR) was then run on 2 ng of Φ29 polymerase amplified and non-amplified DNA in triplicate using a Lightcycler 480 (Roche).

The presence of C-circles was determined by calculating a ∆meanCp value between triplicate Φ29 polymerase amplified and non-amplified runs for each sample.

Senna, which did not amplify for rbcL using Taq polymerase, amplified strongly using the KAPA3G enzyme.

A conventional PCR with a 50°C annealing temperature using high fidelity Taq polymerase, amplified a band of 1457 base pairs.

The taq polymerase amplified segment was inserted into polymerase chain reaction T7 NT-TOPO so that six histidine residues were added to the N terminus.

Taq DNA polymerase amplified PGC-1α promoter fragment −2310 to −1902 was cloned into the T-tailed EcoRV site of yT&A vector to make the yTA (−2310 to −1902 -construct −1902 -construct

Furthermore, we found that removal of contaminating human genomic DNA by DNase-treatment during the purification step increased the sensitivity of the assay, since the Phi29 DNA polymerase amplifies any DNA present in the assay.

The cycle is then repeated, the two double-stranded DNA sequences are again denatured, the primers attach again, the polymerase amplifies, etc.

Digestion of the pENTR/TA with XcmI allows for TA-cloning of the Taq-Polymerase amplified shRNA-expression cassettes.

To accomplish the tetracycline-inducible expression of fused histone 2B-green fluorescent protein (H2B-GFP), we cloned the Taq polymerase-amplified H2B-GFP gene from Addgene into the PCR8/GW/TOPO entry vector and then into the pT-Rex DEST30 destination vector using the Invitrogen Gateway cloning system and Clonase II enzyme mix.

DNA polymerases amplify small fragments more robustly than large fragments.