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Two approaches under isothermal conditions are presented: recombinase polymerase amplification (RPA) and multiple displacement amplification (MDA).
Isothermal amplification was conducted using thermophillic helicase dependant amplification and recombinase polymerase amplification.
An isothermal heterogeneous asymmetric recombinase polymerase amplification (haRPA) was carried out on flow-based CL DNA microarrays.
Recombinase polymerase amplification (RPA), combined with lateral flow (LF) strips, is a novel approach to rapidly amplify and visualize DNA.
In order to remove these substitution errors (possibly due to sequencing errors and/or polymerase amplification errors), we clustered the barcode sequence by using our in-house software, Nucleotide Sequence Clusterizer (see Methods).
The objective of this study was to develop a real-time recombinase polymerase amplification (rt-RPA) assay for the rapid detection of porcine circovirus 3 (PCV3).
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Objectives: to design a generic adenovirus nested polymerase chain amplification assay designed to be capable of detecting all respiratory adenoviruses.
The cells were washed 1× in PBS-T and treated with polymerase for amplification for 90 minutes at 37°C.
The entire immunoprecipitation reaction and 500 ng of control DNA were amplified using the T7 RNA polymerase linear amplification protocol as described [45].
This initial screening procedure identified a preferred polymerase and amplification conditions that showed improved tolerance to AT-rich templates.
In order to activate the polymerase, all amplification programs included an initial denaturation step at 95°C for 15 min.
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